Patrick Van Dijck
The first and largest subgroup is focusing on glucose and amino acid sensing and signal transduction pathways in the human fungal pathogens Candida albicans and Candida glabrata. In order to study these pathways we are developing genome-wide Candida specific protein-protein interaction tools, including FRET based biosensors. We have developed an in vivo subcutaneous rodent model system to study biofilms of Candida sp. We use this model to test novel drugs (e.g. essential oil components), to identify genes involved in biofilm formation and to study the host immune response.Finally, we have identified a possible mechanism that allows the appearance of resistance to azole drugs and we are determining the underlying molecular mechanism. The second subgroup is working on plant trehalose metabolism. On the one hand we want to understand the role of trehalose biosynthesis genes in plant development and stress tolerance. We are using various strategies to use trehalose metabolism in order to generate plants with a better stress tolerance.
- Kucharikova S., Neirinck B., Sharma N., Vleugels J., Lagrou K., Van Dijck P. 2015. In vivo Candida glabrata biofilm development on foreign bodies in a rat subcutaneous model. The Journal of Antimicrobial Chemotherapy. vol.70 (3) , pp. 846-856
- Vande Velde G., Kucharíková S., Schrevens S., Himmelreich U., Van Dijck P. 2014. Towards non-invasive monitoring of pathogen-host interactions during Candida albicans biofilm formation using in vivo bioluminescence. Cellular Microbiology. vol. , pp. 115-130
- Van Houtte H., Vandesteene L., Lopez-Galvis L., Lemmens L., Kissel E., Carpentier S., Feil R., Avonce N., Beeckman T., Lunn J., Van Dijck P. 2013. Over-expression of the trehalase gene AtTRE1 leads to increased drought stress tolerance in Arabidopsis and is involved in ABA-induced stomatal closure. Plant Physiology. vol. , pp. 1158-1171
- Beaussart A., Alsteens D., El-Kirat-Chatel S., Lipke P., Kucharíková S., Van Dijck P., Dufrêne Y. 2012. Single-Molecule Imaging and Functional Analysis of Als Adhesins and Mannans during Candida albicans Morphogenesis. ACS Nano. vol.6 (12) , pp. 10950-10964
- Stynen B., Tournu H., Tavernier J., Van Dijck P. 2012. Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system. Microbiology and Molecular Biology Reviews. vol.76 (2) , pp. 331-382