Publications


Articles in internationally reviewed journals

2012

  • Tobback, J., Boerjan, B., Vandermissen, H.P. and Huybrechts, R. (2012) Male reproduction is affected by RNA interference of period and timeless in the desert locust Schistocerca gregaria. Insect Biochemistry and Molecular Biology, 42(2), 109-115.
  • Boerjan, B.*, Tobback, J*., Vandersmissen, H.P., Huybrechts, R. and Schoofs, L. (2012). Fruitless RNAi knockdown in the desert locust, Schistocerca gregaria, influences male fertility. Journal of Insect Physiology, 58(2), 265-269  (* both authors contributed equally)
     

2011

  • Vuerinckx, K.*, Verlinden, H.*, Lindemans, M., Vanden Broeck, J., Huybrechts, R. (2011). Characterization of an allatotropin-like peptide receptor in the red flour beetle, Tribolium castaneum. Insect Biochemistry and molecular biology, accepted for publication.
  • Tobback, J., Vandersmissen, H.P., Boerjan, B. and Huybrechts, R. (2011). The circadian clock genes affect reproductive capacity in the desert locust Schistocerca gregaria. Insect Biochemistry and molecular biology, in press.
  • Boerjan, B.*, Tobback, J*., De Loof, A., Schoofs, L. and Huybrechts, R. (2011). Fruitless RNAi knockdown in males interferes with copulation success in Schistocerca gregaria. Insect Biochemistry and molecular biology, in press.
  • Badisco,L., Huybrechts,J., Simonet,G., Verlinden,H., Marchal,E., Huybrechts,R., Schoofs,L., De Loof,A., Vanden Broeck,J. (2011). Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database PLoS ONE, in press.
  • Tobback, J.*, Mommaerts, V.*, Vandersmissen, H., Smagghe, G., Huybrechts, R. (2011). Age- and size-dependent foraging gene expression in the bumblebee Bombus terrestris. Archives of Insect Biochemistry and Physiology, 76(1), 30-42.

2010

  • Verlinden, H., Vleugels, R., Marchal, E., Badisco, L., Tobback, J., Pflüger, H., Blenau, W., Vanden Broeck, J. (2010). The cloning, phylogenetic relationship and distribution pattern of two new putative GPCR-type octopamine receptors in the desert locust (Schistocerca gregaria). Journal of Insect Physiology, 56 (8), 868-875.
  • Marchal E, Vandersmissen HP, Badisco L, Van de Velde S, Verlinden H, Iga M, Van Wielendaele P, Huybrechts R, Simonet G, Smagghe G, Vanden Broeck J. (2010) Control of ecdysteroidogenesis in prothoracic glands of insects: a review. Peptides. 2010 Mar;31(3):506-19. Epub 2009 Aug 31. Review.

2009

  • Van Hiel MB, Van Wielendaele P, Temmerman L, Van Soest S, Vuerinckx K, Huybrechts R, Broeck JV, Simonet G. (2009) Identification and validation of housekeeping genes in brains of the desert locust Schistocerca gregaria under different developmental conditions. BMC Mol Biol. 2009 Jun 9;10:56.

2008

  • Gobin B, Heylen K, Billen J, Arckens L, Huybrechts R. (2008) Sublethal effects of crop protection on honey bee pollination: foraging behaviour and flower visits. Commun Agric Appl Biol Sci. 2008;73(3):405-8.
  • J. Tobback, K. Heylen, B. Gobin, T. Wenseleers, J. Billen, L. Arckens & R. Huybrechts (2008) Cloning and expression of PKG, a candidate foraging regulating gene in Vespula vulgaris. Animal Biology 58: 341-351.
  • Heylen K, Gobin B, Billen J, Hu TT, Arckens L, Huybrechts R. (2008) Amfor expression in the honeybee brain: a trigger mechanism for nurse-forager transition. J Insect Physiol. 2008 Oct-Nov;54(10-11):1400-3. Epub 2008 Aug 3

2007

  • Sas, F., M. Begum, T. Vandersmissen, M. Geens, I. Claeys, S. Van Soest, J. Huybrechts, R. Huybrechts & A. De Loof (2007). Development of a real-time PCR assay for measurements of yellow protein mRNA transcription in the desert locust Schistocerca gregaria: A basis for isolation of a peptidergic regulatory factor. Peptides, 28(1): 38-43

2005

  • Breuer M, De Loof A, Balzarini J, Huybrechts R. (2005) Insecticidal activity of the pyrimidine nucleoside analogue (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU). Pest Manag Sci. 2005 Aug;61(8):737-41.

2004

  • Hens K, Lemey P, Macours N, Francis C, Huybrechts R. (2004) Cyclorraphan yolk proteins and lepidopteran minor yolk proteins originate from two unrelated lipase families. Insect Mol Biol. 2004 Dec;13(6):615-23.
  • Hens K, Macours N, Claeys I, Francis C, Huybrechts R. (2004) Cloning and expression of the yolk protein of the tsetse fly Glossina morsitans morsitans. Insect Biochem Mol Biol. 2004 Dec;34(12):1281-7.
  • Macours N, Poels J, Hens K, Francis C, Huybrechts R. (2004) Structure, evolutionary conservation, and functions of angiotensin- and endothelin-converting enzymes. Int Rev Cytol. 2004;239:47-97. Review.
  • Leysen I, Van der Gucht E, Eysel UT, Huybrechts R, Vandesande F, Arckens L. (2004) Time-dependent changes in the expression of the MEF2 transcription factor family during topographic map reorganization in mammalian visual cortex. Eur J Neurosci. 2004 Aug;20(3):769-80.
  • Deloffre L, Sautiere PE, Huybrechts R, Hens K, Vieau D, Salzet M. (2004) Angiotensin-converting enzyme inhibition studies by natural leech inhibitors by capillary electrophoresis and competition assay. Eur J Biochem. 2004 Jun;271(11):2101-6.

2003

  • Macours N, Hens K, Francis C, De Loof A, Huybrechts R. (2003) Molecular evidence for the expression of angiotensin converting enzyme in hemocytes of Locusta migratoria: stimulation by bacterial lipopolysaccharide challenge. J Insect Physiol. 2003 Aug;49(8):739-46.
  • Arckens L, Van der Gucht E, Van den Bergh G, Massie A, Leysen I, Vandenbussche E, Eysel UT, Huybrechts R, Vandesande F. (2003) Differential display implicates cyclophilin A in adult cortical plasticity. Eur J Neurosci. 2003 Jul;18(1):61-75.
  • Rahman MM, Vandingenen A, Begum M, Breuer M, De Loof A, Huybrechts R. (2003) Search for phase specific genes in the brain of desert locust, Schistocerca gregaria (Orthoptera: Acrididae) by differential display polymerase chain reaction. Comp Biochem Physiol A Mol Integr Physiol. 2003 Jun;135(2):221-8.
  • Macours N, Poels J, Hens K, Luciani N, De Loof A, Huybrechts R. (2003) An endothelin-converting enzyme homologue in the locust, Locusta migratoria: functional activity, molecular cloning and tissue distribution. Insect Mol Biol. 2003 Jun;12(3):233-40.

2002

  • Meeusen T, Mertens I, Clynen E, Baggerman G, Nichols R, Nachman RJ, Huybrechts R, De Loof A, Schoofs L. (2002) Identification in Drosophila melanogaster of the invertebrate G protein-coupled FMRFamide receptor. Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15363-8. Epub 2002 Nov 18.
  • Vandingenen A, Hens K, Baggerman G, Macours N, Schoofs L, De Loof A, Huybrechts R. (2002) Isolation and characterization of an angiotensin converting enzyme substrate from vitellogenic ovaries of Neobellieria bullata. Peptides. 2002 Oct;23(10):1853-63.
  • Mertens I, Meeusen T, Huybrechts R, De Loof A, Schoofs L. (2002) Characterization of the short neuropeptide F receptor from Drosophila melanogaster. Biochem Biophys Res Commun. 2002 Oct 11;297(5):1140-8.
  • Hens K, Vandingenen A, Macours N, Baggerman G, Karaoglanovic AC, Schoofs L, De Loof A, Huybrechts R. (2002) Characterization of four substrates emphasizes kinetic similarity between insect and human C-domain angiotensin-converting enzyme. Eur J Biochem. 2002 Jul;269(14):3522-30.
  • Vandingenen A, Hens K, Macours N, Schoofs L, De Loof A, Huybrechts R. (2002) Presence of angiotensin converting enzyme (ACE) interactive factors in ovaries of the grey fleshfly Neobellieria bullata. Comp Biochem Physiol B Biochem Mol Biol. 2002 May;132(1):27-35.

2001

  • Zhu W, Vandingenen A, Huybrechts R, Vercammen T, Baggerman G, De Loof A, Poulos CP, Velentza A, Breuer M. (2001) Proteolytic breakdown of the Neb-trypsin modulating oostatic factor (Neb-TMOF) in the hemolymph of different insects and its gut epithelial transport. J Insect Physiol. 2001 Nov;47(11):1235-1242.
  • Vandingenen A, Hens K, Macours N, Zhu W, Janssen I, Breuer M, De Loof A, Huybrechts R. (2001) Captopril, a specific inhibitor of angiotensin converting enzyme, enhances both trypsin and vitellogenin titers in the grey fleshfly Neobellieria bullata. Arch Insect Biochem Physiol. 2001 Jul;47(3):161-7.
  • Zhu W, Vandingenen A, Huybrechts R, Baggerman G, De Loof A, P Poulos C, Velentza A, Breuer M. (2001) In vitro degradation of the Neb-Trypsin modulating oostatic factor (Neb-TMOF) in gut luminal content and hemolymph of the grey fleshfly, Neobellieria bullata. Insect Biochem Mol Biol. 2001 Jan;31(1):87-95.

2000

  • Balzarini J, Degrève B, Hatse S, De Clercq E, Breuer M, Johansson M, Huybrechts R, Karlsson A. (2000) The multifunctional deoxynucleoside kinase of insect cells is a target for the development of new insecticides. Mol Pharmacol. 2000 Apr;57(4):811-9.

1980-1999

  • De Loof A, Huybrechts R, Kotanen S. Reproduction and love: strategies of the organism's cellular defense system? Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1998 Aug;120(2):167-76. Review.
  • Wenseleers T, Ito F, Van Borm S, Huybrechts R, Volckaert F, Billen J. Widespread occurrence of the micro-organism Wolbachia in ants. Proc Biol Sci. 1998 Aug 7;265(1404):1447-52.
  • De Loof A, Huybrechts R. "Insects do not have sex hormones": a myth? Gen Comp Endocrinol. 1998 Sep;111(3):245-60. Review.
  • Schoofs L, Veelaert D, De Loof A, Huybrechts R, Isaac E. Immunocytochemical distribution of angiotensin I-converting enzyme-like immunoreactivity in the brain and testis of insects. Brain Res. 1998 Mar 2;785(2):215-27.
  • Borovsky D, Janssen I, Vanden Broeck J, Huybrechts R, Verhaert P, De Bondt HL, Bylemans D, De Loof A. Molecular sequencing and modeling of Neobellieria bullata trypsin. Evidence for translational control by Neobellieria trypsin-modulating oostatic factor. Eur J Biochem. 1996 Apr 1;237(1):279-87.
  • Vanden Broeck J, Vulsteke V, Huybrechts R, De Loof A. Characterization of a cloned locust tyramine receptor cDNA by functional expression in permanently transformed Drosophila S2 cells. J Neurochem. 1995 Jun;64(6):2387-95.
  • De Loof A, Bylemans D, Schoofs L, Janssen I, Spittaels K, Vanden Broeck J, Huybrechts R, Borovsky D, Hua YJ, Koolman J, et al. Folliculostatins, gonadotropins and a model for control of growth in the grey fleshfly, Neobellieria (sarcophaga) bullata. Insect Biochem Mol Biol. 1995 Jun;25(6):661-7.
  • Bylemans D, Proost P, Samijn B, Borovsky D, Grauwels L, Huybrechts R, Van Damme J, Van Beeumen J, De Loof A. Neb-colloostatin, a second folliculostatin of the grey fleshfly, Neobellieria bullata. Eur J Biochem. 1995 Feb 15;228(1):45-9.
  • Vandenbroeck K, Willems L, Billiau A, Opdenakker G, Huybrechts R. Glycoform heterogeneity of porcine interferon-gamma expressed in Sf9 cells. Lymphokine Cytokine Res. 1994 Aug;13(4):253-8.
  • Vulsteke V, Janssen I, Vanden Broeck J, De Loof A, Huybrechts R. Baculovirus immediate early gene promoter based expression vectors for transient and stable transformation of insect cells. Insect Mol Biol. 1993;2(4):195-204.
  • Huybrechts R, Guarino L, Van Brussel M, Vulsteke V. Nucleotide sequence of a transactivating Bombyx mori nuclear polyhedrosis virus immediate early gene. Biochim Biophys Acta. 1992 Feb 11;1129(3):328-30.
  • Clottens F, Gäde G, Huybrechts R, De Loof A. Immunohistochemical localisation of the hypertrehalosaemic hormone II (Cam-HrTH-II) and related peptides in the nervous system of Carausius morosus and Sarcophaga bullata. Cell Tissue Res. 1989 Dec;258(3):631-6.
  • Verhaert PD, Downer RG, Huybrechts R, De Loof A. A substance resembling somatomedin C in the American cockroach. Regul Pept. 1989 Apr;25(1):99-110.
  • Schoofs L, Danger JM, Jegou S, Pelletier G, Huybrechts R, Vaudry H, De Loof A. NPY-like peptides occur in the nervous system and midgut of the migratory locust, Locusta migratoria and in the brain of the grey fleshfly, Sarcophaga bullata. Peptides. 1988 Sep-Oct;9(5):1027-36.
  • Schoofs L, Jégou S, Tonon MC, Huybrechts R, Vaudry H, De Loof A. Biological, chromatographical, and radioimmunological evidence for a melanotropin-like peptide in the central nervous system of Locusta migratoria. Gen Comp Endocrinol. 1988 Jul;71(1):36-44.
  • Schoofs L, Jegou S, Andersen AC, Tonon MC, Eberle AN, Huybrechts R, De Loof A, Vaudry H. Coexistence of melanin-concentrating hormone and alpha-melanocyte-stimulating hormone immunoreactivities in the central nervous system of the locust, Locusta migratoria. Brain Res. 1988 May 31;450(1-2):202-8.
  • Schoofs L, Schrooten S, Huybrechts R, De Loof A. Methionine-enkephalin immunoreactivity in the gonads and nervous system of two insect species: Locusta migratoria and Sarcophaga bullata. Gen Comp Endocrinol. 1988 Jan;69(1):1-12.
  • Schols D, Verhaert P, Huybrechts R, Vaudry H, Jégou S, De Loof A. Immunocytochemical demonstration of proopiomelanocortin- and other opioid-related substances and a CRF-like peptide in the gut of the american cockroach, Periplaneta americana L. Histochemistry. 1987;86(4):345-51.
  • Verhaert P, De Loof A, Huybrechts R, Delang I, Theunis W, Clottens F, Schoofs L, Swinnen K, Vandesande F. A new alternative for simultaneous immunohistochemical screening of 96 hybridoma clones for tissue-specific antibody productions selects a monoclonal antibody to insect corpus cardiacum. J Neurosci Methods. 1986 Sep;17(4):261-8.
  • Stoppie P, Briers T, Huybrechts R, De Loof A. Moulting hormone, juvenile hormone and the ultrastructure of the fat body of adult Sarcophaga bullata (Diptera). Cell Tissue Res. 1981;221(2):233-44. 

 
Articles in other journals

  • Beliën, T., Kellers, J., Heylen, K., Keulemans, W., Billen, J., Arckens, L., Huybrechts, R., Gobin, B. (2009). Effects of sublethal doses of crop protection agents on honey bee (Apis mellifera) global colony vitality and its potential link with aberrant foraging activity. Communications in Agricultural and Applied Biological Sciences, 74(1), 245-253.
  • Gobin, B., Heylen, K., Billen, J., Arckens, L., Huybrechts, R. (2008). Sublethal effects of crop protection on honey bee pollination: foraging behaviour and flower visits. Communications in Agricultural and Applied Biological Sciences, 73(3), 405-8.


Other published books; as author

  • Huybrechts, R. (2008). Vergelijkende biologie voor de medische en biomedische wetenschappen: tekstboek. Leuven, Belgium: Acco.
  • Huybrechts, R. (2008). Vergelijkende biologie voor de medische en biomedische wetenschappen: boek met illustraties. Leuven, Belgium: Acco.


Papers at international conferences and symposia, published in full in proceedings

  • Gobin, B., Heylen, K., Billen, J., Huybrechts, R., Arckens, L. (2008). Sub-lethal exposure of honey bees to crop-protection: feeding behaviour and flower visits. In : IOBC/WPRS Bulletin. Proceedings of Workshop on Integrated Soft Fruit Production., 39, (Linder, C., Cross, J. (Eds.)). Workshop on Integrated Soft Fruit Production. East Malling, UK, 24-27 September 2007 (pp. 155-159).


Meeting abstracts, presented at international conferences and symposia, published or not published in proceedings or journals

  • Tobback, J., Mommaerts, V., Vandersmissen, H., Smagghe, G., Huybrechts, R. (2010) The foraging gene in Bombus terrestris: Effect of age, task and insecticides. Bee-togheter meeting, Gent (Belgium) 21 december 2010.
  • Verlinden, H., Vleugels, R., Vuerinckx, K., Schoofs, L., Vanden Broeck, J., Huybrechts, R. (2010). Characterisation of orexin receptor orthologues in insects. IAP 6/14, Barcelona, 5-6 November 2010.
  • Tobback, J., Mommaerts, V., Vandersmissen, H., Smagghe, G., Huybrechts, R. (2010). Age and task dependent foraging gene expression in the bumblebee bombus terrestris, abstr.nr. 15-17. IUSSI, Copenhagen, 8-14 august 2010.
  • Vuerinckx, K., Huybrechts, R. (2010). Feeding and starvation effects on the overall endocrinological regulation with special emphasis towards reproduction and circadian clock regulation, abstr.nr. Poster. 7th International Congress of Neuroendocrinology, Rouen, France, 11-15 July 2010.
  • Huybrechts, R., Vuerinckx, K., Tobback, J., Verlinden, H., Lindemans, M. (2010). The orexine receptor pathway in insects. 17th Benelux Congress of Zoology - Classic Biology in Modern Times, Ghent, Belgium, 22-23 October 2010.
  • Beliën, T., Kellers, J., Heylen, K., Billen, J., Arckens, L., Huybrechts, R., Gobin, B. (2009). Identification and evaluation of sublethal effects of crop protection products on honey bees (Apis mellifera). WG Meeting “Pesticides and Beneficial Organisms” of the IOBC, Dubrovnik (Croatia), 7-9 October 2009.
  • Beliën, T., Kellers, J., Heylen, K., Keulemans, W., Billen, J., Arckens, L., Huybrechts, R., Gobin, B. (2009). Impact of sublethal doses of crop protection agents on honey bees: aberrant onset of foraging and its influence on global colony vitality . In : Communications in Agricultural and Applied Biological Sciences. International Symposium on Crop Protection, Gent, Belgium, May 19, 2009 Universiteit Gent.
  • Vuerinckx, K., Tobback, J., Bongaers, I., Huybrechts, R. (2008). Expression of the malvolio gene in the adult brain of the social wasp Vespula vulgaris. Benelux Congress of Zoology, Liege, Belgium, 30 - 31 october.
  • Tobback, J., Bongaers, I., Vandersmissen, H., Huybrechts, R. (2008). Cloning and expression of the foraging gene in the bumblebee Bombus terrestris. IUSSI European Meeting, La Roche, Belgium, 30 august - 4 september.
  • Heylen, K., Billen, J., Huybrechts, R. (2008). An integrated molecular and histological study on the impact of sublethal effects of Fenoxycarb on the European honeybee, Apis mellifera. The International Congress of Entomology, Durban, South Africa, July 2008.
  • Gobin, B., Heylen, K., Billen, J., Arckens, L., Huybrechts, R. (2008). Sublethal effects of crop protection on honey bee pollination: foraging behaviour and flower visits. The International Congress of Entomology, Durban, South Africa, July 6-12, 2008.
  • Gobin, B., Heylen, K., Billen, J., Arckens, L., Huybrechts, R. (2008). Sublethal effects of crop protection on honey bee pollination: foraging behaviour, flower visits and an evaluation of molecular characterization. International Symposium on crop protection, Gent, Belgium, May 20, 2008.
  • Huybrechts, R. (2008). Regulation of behavior is mostly polygenic. Benelux bees symposium edition, Leuven, May 2008.
  • Tobback, J., Huybrechts, R. (2007). Circadian rhythm in the desert locust Schistocerca gregaria. Benelux Congress of Zoology, Amsterdam, The Netherlands, 1 - 2 november.
  • Vanden Broeck, J., Huybrechts, R., Schoofs, L., De Loof, A. (2007). The study of locust peptides as molecular markers of phase transition. Invertebrate Neuropeptide Conference INC2007, Luang Prabang, Laos, 14th-19th January, 2007.
  • Heylen, K., Gobin, B., Billen, J., Arckens, L., Huybrechts, R. (2006). Development of a real-time RT-PCR assay to quantify the honeybee’s (Apis mellifera) Amfor gene expression. Benelux Congress of Zoology, Leuven, Belgium, October 27-28, 2006.
  • Francis, C., Tobback, J., Ceulemans, L., Wenseleers, T., Billen, J., Huybrechts, R. (2006). Food seeking behaviour and for gene expression. Benelux Congress of Zoology, Leuven, Belgium, 27 - 28 october.
  • Sas, F., Begum, M., Vandersmissen, T., Claeys, I., Van Soest, S., Huybrechts, J., Huybrechts, R., De Loof, A. (2006). Yellow coloration in the desert locust Schistocerca gregaria: a realtime-PCR-based bioassay. Conference of European Comparative Endocrinologists (CECE), Manchester, U.K., 29th August – 2nd September, 2006.


Meeting abstracts, presented at other conferences and symposia, published or not published in proceedings or journals

  • Huybrechts, R., Vuerinckx, K., Verlinden, H., Lindemans, M. (2010). Sexual dimorphism in the fat body and gonadal orexine receptor expression of the storage beetle Tribolium castaneum, abstr.nr. Poster. One-Day Symposium on Chemical Entomology, Gembloux, Belgium, 5 May 2010.
  • Huybrechts, R. (2006). Biology in the postgenomic era: scientific realizations and ethical considerations. Dondeyne Stichting, Dondeyne Stichting, Dondeyne Stichting, Dondeyne Stichting, Zoological Institute, Leuven, Zoological Institute, Leuven, Zoological Institute, Leuven, Zoological Institute, Leuven, November 2006November 2007October 2008October 2009.


Thesis

  • Tobback, J., Huybrechts, R. (sup.) (2011). Exploring candidate genes regulating polyethism, phase transition and reproductive success in insects.
  • Heylen, K., Arckens, L. (sup.), Huybrechts, R. (sup.), Gobin, B. (cosup.) (2009). Side effects of crop protection products on bees: identification and evaluation of the impact of sublethal effects..
  • Francis, C., Huybrechts, R. (sup.), Callaerts, P. (cosup.) (2007). Molecular-genetic analysis of Drosophila melanogaster neprilysin 1-5 and Locusta migratoria ECE, 164 pp.